Journal: Oncotarget

Article Title: Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

doi: 10.18632/oncotarget.21211

Figure Lengend Snippet: Representative photographs of the acellular BAMG-SF scaffold (BAMG, SF and cross-sectional views) (A) and the ASCs-seeded BAMG-SF scaffold from the porous SF side (B) , the waterproof BAMG side (C) and the bilayer cross-sectional view (D) . Representative SEM images displaying ASCs inside the SF layer (E) , the microstructure of porous SF layer (F) and BAMG layer (G) and the cross-sectional view (H) . (I) ASCs spread across the whole SF layer after a 3-day incubation in vitro , as shown by H&E stain. (J) ASCs were present as separate cells or cell clusters inside the SF layer under immunofluorescence, as shown by CM-DiI (red) labelling and DAPI (blue) counterstaining. (K) The BAMG-SF-conditioned media did not alter the proliferation of ASCs significantly within 1 week in vitro , suggesting the good cytocompatibility of BAMG-SF. (L) Incubation with ASCs for 3 days had no significant influence on the elastic modulus or maximum load of BAMG-SF. Gross view scale bar = 1 cm; SEM scale bar = 100 μm (E) ; SEM scale bar = 500 μm (F-H) ; H&E scale bar = 100 μm; immunofluorescence scale bar = 50 μm. Data are expressed as the mean ± standard deviation. Cell proliferation experiments and mechanical tests were performed in triplicate.

Article Snippet: Zambon et al. could not conclude whether Vybrant CM-DiI-labelled ASCs, muscle-derived stem cells and α-SMA + smooth muscle cells are of the same origin [ ].

Techniques: Incubation, In Vitro, Staining, Immunofluorescence, Standard Deviation

Journal: Oncotarget

Article Title: Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

doi: 10.18632/oncotarget.21211

Figure Lengend Snippet: Body weight change and bladder calculus of SD rats at different timepoints

Article Snippet: Zambon et al. could not conclude whether Vybrant CM-DiI-labelled ASCs, muscle-derived stem cells and α-SMA + smooth muscle cells are of the same origin [ ].

Techniques:

Journal: Oncotarget

Article Title: Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

doi: 10.18632/oncotarget.21211

Figure Lengend Snippet: Blood cell counts and serum biochemistry of SD rats at different timepoints

Article Snippet: Zambon et al. could not conclude whether Vybrant CM-DiI-labelled ASCs, muscle-derived stem cells and α-SMA + smooth muscle cells are of the same origin [ ].

Techniques:

Journal: Oncotarget

Article Title: Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

doi: 10.18632/oncotarget.21211

Figure Lengend Snippet: Representative gross photographs of regenerated bladder tissue in vivo with ASCs-seeded BAMG-SF at 2 (A) , 4 (B) and 12 (C) weeks and acellular BAMG-SF (D) and cystotomy (E) at 12 weeks. Representative retrograde cystography photographs of bladder augmented by ASCs-seeded BAMG-SF at 2 (F) , 4 (G) and 12 (H) weeks and acellular BAMG-SF (I) and cystotomy (J) at 12 weeks. Black arrows mark the remaining sutures and regenerated tissue present in vivo within the original implantation sites supported by scaffolds. White arrows note depression of the regenerated area under retrograde cystography. Scale bar = 1 cm.

Article Snippet: Zambon et al. could not conclude whether Vybrant CM-DiI-labelled ASCs, muscle-derived stem cells and α-SMA + smooth muscle cells are of the same origin [ ].

Techniques: In Vivo

Journal: Oncotarget

Article Title: Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

doi: 10.18632/oncotarget.21211

Figure Lengend Snippet: (A) Representative immunofluorescence images of the urothelium marker CK, the smooth contractile muscle markerα-SMA, the neuronal marker NeuN, and the blood vessel endothelial marker CD31. Positive respective marker expression is shown in green (labelled by FITC), and the nuclei are counterstained by DAPI (blue); 200×, scale bar = 100 μm. Histomorphometric quantitative comparison of (B) CK + urothelium, (C) α-SMA + smooth muscle bundles, (D) NeuN + neuronal boutons, (E) the mean number per mm 2 and (F) the mean diameter of CD31 + vessels. UE: urothelium; SM: smooth muscle. Typical CM-DiI-labelled ASCs (red) are denoted by white triangles and displayed in magnified inserts; these cells could be detected throughout the 12-week regeneration period. White arrows note NeuN + neuron boutons. “V” marks CD31 + blood vessels. Data are expressed as the mean ± standard deviation. *P<0.05, ***P<0.001 versus the cystotomy group; #P<0.05 versus the BAMG-SF group.

Article Snippet: Zambon et al. could not conclude whether Vybrant CM-DiI-labelled ASCs, muscle-derived stem cells and α-SMA + smooth muscle cells are of the same origin [ ].

Techniques: Immunofluorescence, Marker, Expressing, Comparison, Standard Deviation

Journal: Oncotarget

Article Title: Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

doi: 10.18632/oncotarget.21211

Figure Lengend Snippet: (A) Representative cytograms of the groups augmented with ASCs-seeded BAMG-SF, acellular BAMG-SF and cystotomy at 12 weeks. (B) Residual volume, void volume, and bladder capacity at 12 weeks. (C) Bladder basal, threshold, and peak pressures at 12 weeks. (D) Bladder compliance at 12 weeks. All experiments were performed in triplicate. Data are expressed as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001 versus the cystotomy group; ###P<0.001 versus the BAMG-SF group.

Article Snippet: Zambon et al. could not conclude whether Vybrant CM-DiI-labelled ASCs, muscle-derived stem cells and α-SMA + smooth muscle cells are of the same origin [ ].

Techniques: Standard Deviation

Journal: Oncotarget

Article Title: Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

doi: 10.18632/oncotarget.21211

Figure Lengend Snippet: (A) The transcriptional levels of SDF-1α, CXCR4, VEGF and VEGR2 were significantly elevated in bladder augmented with ASCs-seeded BAMG-SF compared with bladder augmented with acellular BAMG-SF. (B) ASCs seeded in BAMG-SF enhanced SDF-1α, CXCR4, VEGF and VEGFR2 protein expression levels in regenerated bladder tissue. (C) The protein expression levels of AKT and ERK 1/2 were similar among all three groups. An obvious increase in phosphorylated ERK 1/2, but not phosphorylated AKT, was observed in the ASCs-seeded BAMG-SF group. (D) A schematic illustration of the study design and enhanced bladder regeneration facilitated by ASCs-seeded BAMG-SF. Data are expressed as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001 versus the cystotomy group; #P<0.05, versus the BAMG-SF group.

Article Snippet: Zambon et al. could not conclude whether Vybrant CM-DiI-labelled ASCs, muscle-derived stem cells and α-SMA + smooth muscle cells are of the same origin [ ].

Techniques: Expressing, Standard Deviation

Journal: Oncotarget

Article Title: Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

doi: 10.18632/oncotarget.21211

Figure Lengend Snippet: The bladders were immobilized outside the peritoneal cavity (A, E) and subjected to a longitudinal incision of approximately 1 cm in size in the bladder apex. (B, F) . In the augmentation groups, the ASCs-seeded BAMG-SF scaffold (10×10 mm) was incorporated into the bladder defect by fixation of the four corners using non-absorbable 5-0 sutures as markers of the regenerated area and then anastomosed into the bladder defect using continuous absorbable 8-0 sutures (C, D) . The cystotomy group only received the bladder incision, which was immediately closed using sutures (G, H) . Scale bar = 1 cm. Dashed lines mark the incision area. Black triangles indicate the scaffold. Black arrows note the marking sutures between the native bladder and the ASCs-seeded BAMG-SF scaffold.

Article Snippet: Zambon et al. could not conclude whether Vybrant CM-DiI-labelled ASCs, muscle-derived stem cells and α-SMA + smooth muscle cells are of the same origin [ ].

Techniques: